Definition/General

Introduction:
-Eosinophilic gastritis is a rare inflammatory disorder characterized by eosinophilic infiltration of the gastric wall
-It is part of eosinophilic gastroenteritis spectrum
-It involves allergic hypersensitivity mechanisms
-It can affect any layer of the gastric wall.
Origin:
-Results from type I hypersensitivity reaction to food allergens or environmental triggers
-Involves IgE-mediated and non-IgE-mediated mechanisms
-Th2-mediated immune response predominates
-Releases eosinophil-derived mediators
-Causes tissue damage and inflammation.
Classification:
-Classified by predominant layer involvement: mucosal type (most common, 90%)
-Muscular type (gastric outlet obstruction)
-Serosal type (eosinophilic ascites)
-Mixed patterns possible
-Part of eosinophilic gastroenteritis spectrum.
Epidemiology:
-Rare condition (<1% of gastritis cases)
-Male predominance (2:1 ratio)
-Bimodal age distribution: children and young adults
-Associated with atopic conditions (40-50% cases)
-Family clustering reported
-Higher in developed countries.

Clinical Features

Presentation:
-Epigastric pain (most common)
-Nausea and vomiting
-Food allergies and intolerances
-Early satiety and bloating
-Gastric outlet obstruction (muscular involvement)
-Iron deficiency anemia (mucosal ulceration).
Symptoms:
-Abdominal pain (postprandial)
-Dyspepsia and indigestion
-Nausea and vomiting
-Diarrhea (if small bowel involved)
-Weight loss
-Allergic symptoms: rash, asthma, rhinitis
-Protein-losing enteropathy (severe cases).
Risk Factors:
-Food allergies (milk, eggs, seafood, nuts)
-Atopic conditions: asthma, eczema, allergic rhinitis
-Family history of allergies
-Environmental allergens
-Drug hypersensitivity
-Parasitic infections (differential diagnosis).
Screening:
-Peripheral eosinophilia (>4% or >450 cells/μL)
-Elevated IgE levels
-Food-specific IgE testing
-Stool examination (parasites)
-Upper endoscopy with biopsy
-CT scan (muscular/serosal involvement).

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Gross Description

Appearance:
-Mucosal erythema and edema
-Nodular gastritis pattern
-Mucosal thickening and rigidity
-Ulcerations may be present
-Pyloric stenosis (muscular involvement)
-Normal appearance possible (early disease).
Characteristics:
-Antral involvement most common
-Diffuse mucosal changes
-Cobblestone appearance
-Loss of normal fold pattern
-Thickened gastric wall (muscular type)
-Luminal narrowing possible.
Size Location:
-Antral predominance (60-70% cases)
-May involve entire stomach
-Pyloric involvement common
-Concurrent duodenal involvement
-Segmental distribution possible.
Multifocality:
-Patchy distribution initially
-May become diffuse over time
-Often part of eosinophilic gastroenteritis
-Concurrent small bowel involvement
-Skip lesions possible.

Microscopic Description

Histological Features:
-Marked eosinophilic infiltration (>30 eosinophils/hpf)
-Involves lamina propria predominantly
-Chronic inflammation with lymphocytes and plasma cells
-Surface epithelial damage
-Crypt architectural distortion.
Cellular Characteristics:
-Dense eosinophilic infiltrate
-Degranulating eosinophils
-Tissue eosinophilia
-Charcot-Leyden crystals
-Mixed inflammatory infiltrate
-Increased mast cells
-Epithelial damage and regeneration.
Architectural Patterns:
-Mucosal thickening
-Crypt elongation and distortion
-Surface epithelial erosions
-Lamina propria expansion
-Muscular layer involvement (muscular type)
-Submucosal fibrosis (chronic cases).
Grading Criteria:
-Eosinophil count: >30/hpf (mucosal)
->50/hpf (diagnostic threshold)
-Distribution assessment: mucosal, muscular, serosal
-Architectural changes: mild, moderate, severe
-Associated tissue damage evaluation.

Immunohistochemistry

Positive Markers:
-CD68 (increased macrophages)
-Tryptase (increased mast cells)
-CD117 (mast cells)
-Ki-67 (increased proliferation)
-IgE (tissue deposition)
-CD20 (B-cell component).
Negative Markers:
-CD30 (excludes lymphoma)
-ALK (excludes inflammatory myofibroblastic tumor)
-CD117 in spindle cells (excludes GIST)
-Parasitic organisms (special stains negative).
Diagnostic Utility:
-Confirms eosinophilic nature of infiltrate
-Excludes parasitic infection
-Identifies mast cell involvement
-Assesses tissue IgE deposition
-Rules out malignancy
-Evaluates inflammatory response.
Molecular Subtypes:
-IgE-mediated type: immediate hypersensitivity
-Non-IgE-mediated: delayed hypersensitivity
-Mixed pattern
-Th2-driven inflammation
-IL-5 and IL-13 mediated eosinophil recruitment.

Molecular/Genetic

Genetic Mutations:
-STAT6 gene variants
-IL-13 gene polymorphisms
-IL-5 receptor variants
-Eotaxin gene polymorphisms
-FIP1L1-PDGFRA fusion (hypereosinophilic syndrome)
-TPSAB1 gene variants.
Molecular Markers:
-Elevated IL-5 and IL-13
-Increased eotaxin levels
-IgE elevation
-Tryptase levels
-Eosinophil cationic protein
-Th2 cytokine profile.
Prognostic Significance:
-Mucosal type: better prognosis
-Muscular type: risk of obstruction
-Serosal type: risk of ascites
-Response to treatment: varies by subtype
-Chronic fibrosis: poor outcome.
Therapeutic Targets:
-Corticosteroids: first-line therapy
-Elimination diet: food allergen identification
-Antihistamines
-Mast cell stabilizers
-Anti-IL-5 therapy: mepolizumab
-Leukotriene receptor antagonists.

Differential Diagnosis

Similar Entities:
-Parasitic gastritis (Anisakis, Strongyloides)
-Drug-induced eosinophilia
-Hypereosinophilic syndrome
-Inflammatory myofibroblastic tumor
-GIST with eosinophilia
-Crohn disease.
Distinguishing Features:
-Eosinophilic gastritis: tissue eosinophilia >30/hpf, food allergies
-Parasitic: organisms identified, travel history
-Drug-induced: temporal relationship
-HES: systemic eosinophilia >1500/μL
-IMT: spindle cells, ALK+.
Diagnostic Challenges:
-Establishing eosinophil threshold
-Identifying underlying triggers
-Distinguishing from secondary eosinophilia
-Correlating with clinical symptoms
-Excluding parasitic infections
-Assessing extent of involvement.
Rare Variants:
-Eosinophilic ascites (serosal type)
-Gastric perforation
-Massive gastric wall thickening
-Concurrent eosinophilic esophagitis
-Protein-losing gastropathy.

Sample Pathology Report

Template Format

Sample Pathology Report

Complete Report: This is an example of how the final pathology report should be structured for this condition.

Specimen Information

Gastric biopsy from [site], [number] fragments

Diagnosis

Eosinophilic gastritis

Eosinophil Count

Eosinophils: [number]/hpf (normal <20/hpf)

Distribution

Predominant involvement: [mucosal/muscular/serosal]

Histological Features

Dense eosinophilic infiltrate with chronic inflammation and epithelial damage

Parasites

No parasitic organisms identified

Special Studies

Tryptase: [result], CD68: [result]

Peripheral eosinophilia: [present/absent]

[other study]: [result]

Recommendations

Clinical correlation, allergy testing, consider elimination diet

Final Diagnosis

Eosinophilic gastritis with [degree] eosinophilic infiltration