Definition/General

Introduction:
-Small intestinal leiomyoma is a benign smooth muscle tumor arising from muscularis mucosae or muscularis propria
-It represents true smooth muscle neoplasm
-It shows low mitotic activity and no cellular atypia
-It is less common than gastric leiomyomas.
Origin:
-Arises from smooth muscle cells of intestinal wall
-Most commonly from muscularis propria
-Can originate from muscularis mucosae
-Results from benign proliferation of smooth muscle cells
-Hormonal influences may play role.
Classification:
-Based on size: small (<2 cm), large (>2 cm)
-Based on location: intramural, subserosal, submucosal
-Solitary (most common) vs multiple
-Associated with hereditary leiomyomatosis (rare).
Epidemiology:
-Rare tumors (<5% of small bowel neoplasms)
-Female predominance (2:1 ratio)
-Peak incidence: 40-60 years
-Jejunum most common location (50%)
-Higher incidence than leiomyosarcoma.

Clinical Features

Presentation:
-Often asymptomatic (small tumors)
-Abdominal pain (most common symptom)
-Small bowel obstruction (20-30% cases)
-GI bleeding (if mucosal ulceration)
-Palpable mass (large tumors).
Symptoms:
-Crampy abdominal pain
-Intestinal obstruction symptoms
-Occult GI bleeding (iron deficiency anemia)
-Early satiety (large tumors)
-Nausea and vomiting
-Weight loss (obstruction cases).
Risk Factors:
-Female sex (hormonal influence)
-Reproductive age
-Multiple leiomyomatosis syndrome
-Hereditary leiomyomatosis and renal cell carcinoma (HLRCC)
-Previous pelvic surgery.
Screening:
-CT abdomen (well-circumscribed mass)
-MRI (T2 hypointense)
-Upper endoscopy (duodenal lesions)
-Capsule endoscopy
-Small bowel follow-through
-EUS (wall layer assessment).

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Gross Description

Appearance:
-Well-circumscribed mass
-Firm, rubbery consistency
-Gray-white cut surface
-Whorled appearance
-Smooth surface
-Encapsulated or pseudoencapsulated.
Characteristics:
-Intramural location most common
-Smooth, lobulated contour
-Homogeneous cut surface
-Fascicular pattern visible
-No necrosis or hemorrhage typically
-Well-demarcated from surrounding tissue.
Size Location:
-Jejunum (50% cases)
-Ileum (30%)
-Duodenum (20%)
-Size range: 1-15 cm (average 3-5 cm)
-Solitary lesions (90%)
-Intramural growth pattern.
Multifocality:
-Usually solitary
-Multiple leiomyomas (hereditary syndromes)
-Associated uterine leiomyomas common
-Concurrent with other GI tract leiomyomas
-Bilateral renal involvement (HLRCC syndrome).

Microscopic Description

Histological Features:
-Spindle-shaped smooth muscle cells
-Fascicular arrangement
-Interlacing bundles
-Elongated nuclei with blunt ends
-Eosinophilic cytoplasm
-Low mitotic activity (<2 mitoses per 10 HPF).
Cellular Characteristics:
-Uniform spindle cells
-Cigar-shaped nuclei
-No nuclear atypia
-Minimal pleomorphism
-Abundant eosinophilic cytoplasm
-Longitudinal striations visible
-Low mitotic rate.
Architectural Patterns:
-Interlacing fascicles
-Whorled pattern
-Perpendicular orientation of fascicles
-Hyalinized areas possible
-Calcifications rare
-No necrosis typically.
Grading Criteria:
-Mitotic count: <2 per 10 HPF (diagnostic criterion)
-Nuclear atypia: absent or minimal
-Cellularity: moderate
-Necrosis: absent
-Size: variable significance.

Immunohistochemistry

Positive Markers:
-Desmin (positive, diagnostic)
-Smooth muscle actin (SMA, positive)
-Caldesmon (positive)
-Vimentin (positive)
-h-Caldesmon (high molecular weight, specific).
Negative Markers:
-CD117 (KIT) (negative, excludes GIST)
-DOG1 (negative, excludes GIST)
-S-100 (negative, excludes schwannoma)
-CD34 (negative)
-Chromogranin A (negative).
Diagnostic Utility:
-Confirms smooth muscle differentiation
-Desmin positivity diagnostic for smooth muscle
-Distinguishes from GIST (CD117-negative)
-Excludes nerve sheath tumors (S-100 negative)
-Confirms benign nature.
Molecular Subtypes:
-Conventional leiomyoma: typical smooth muscle markers
-Epithelioid variant: round cell morphology but same markers
-Hereditary forms: fumarate hydratase deficiency.

Molecular/Genetic

Genetic Mutations:
-FH gene mutations (hereditary leiomyomatosis)
-Chromosomal rearrangements
-12q14-15 deletions
-HMGA2 rearrangements
-MED12 mutations (some cases).
Molecular Markers:
-Fumarate hydratase deficiency (HLRCC)
-2-Succinocysteine accumulation
-Smooth muscle-specific proteins
-Low Ki-67 proliferation index
-Normal p53 expression.
Prognostic Significance:
-Benign behavior: no metastatic potential
-Complete excision: curative
-Size >5 cm: higher recurrence risk if incomplete excision
-Multiple tumors: suggest genetic syndrome.
Therapeutic Targets:
-Complete surgical excision
-Enucleation (small tumors)
-Segmental resection (large tumors)
-Laparoscopic approach possible
-Genetic counseling (multiple tumors).

Differential Diagnosis

Similar Entities:
-Gastrointestinal stromal tumor (GIST)
-Leiomyosarcoma
-Schwannoma
-Inflammatory myofibroblastic tumor
-Fibroma
-Solitary fibrous tumor.
Distinguishing Features:
-Leiomyoma: desmin+, CD117-, low mitoses
-GIST: CD117+, DOG1+
-Leiomyosarcoma: high mitoses (>5/10 HPF), atypia
-Schwannoma: S-100+
-IMT: ALK+ (50% cases).
Diagnostic Challenges:
-Distinguishing from low-grade leiomyosarcoma
-Mitotic count accuracy
-Identifying epithelioid variant
-Excluding GIST (immunohistochemistry essential)
-Assessing margin status.
Rare Variants:
-Epithelioid leiomyoma
-Leiomyoblastoma (outdated term)
-Cellular leiomyoma
-Hyalinized leiomyoma
-Multiple intestinal leiomyomatosis.

Sample Pathology Report

Template Format

Sample Pathology Report

Complete Report: This is an example of how the final pathology report should be structured for this condition.

Specimen Information

Small bowel resection with tumor, measuring [size] cm

Diagnosis

Small intestinal leiomyoma

Histological Features

Well-circumscribed spindle cell tumor with smooth muscle differentiation

Mitotic Activity

Mitotic count: [X] per 10 high-power fields (normal <2/10 HPF)

Size and Location

Tumor size: [X] cm, location: [intramural/subserosal/submucosal]

Margins

Resection margins: [uninvolved/involved], closest margin: [X] mm

Special Studies

Desmin: positive, SMA: positive, CD117: negative, DOG1: negative

Ki-67 proliferation index: [low percentage]

[other study]: [result]

Final Diagnosis

Small intestinal leiomyoma, [size] cm, with clear margins