Overview

Definition:
-Fragile X syndrome (FXS) is an inherited genetic disorder that causes intellectual disability, developmental delays, and behavioral and learning challenges
-It is the most common inherited cause of intellectual disability and a leading genetic cause of autism spectrum disorder.
Epidemiology:
-FXS affects approximately 1 in 4,000 to 5,000 males and 1 in 8,000 to 10,000 females
-The prevalence is consistent across different ethnic groups
-Carrier rates for premutation alleles are higher, with about 1 in 250 females and 1 in 500 males carrying a premutation allele.
Clinical Significance:
-Understanding FXS is crucial for pediatricians to ensure early diagnosis, implement appropriate developmental support services, manage associated comorbidities, and provide genetic counseling to affected families
-Early intervention significantly improves long-term outcomes.

Clinical Presentation

Symptoms:
-Developmental delays in reaching milestones like sitting and walking
-Intellectual disability, ranging from mild to severe
-Speech and language problems, including delayed speech, difficulties with articulation, and stuttering
-Attention deficit hyperactivity disorder (ADHD)
-Autistic behaviors, such as poor eye contact, repetitive movements, and social interaction challenges
-Physical features, which can become more apparent with age: long face, prominent jaw, large ears, broad forehead, and postpubertal macroorchidism in males
-Seizures occur in approximately 10-15% of individuals with FXS.
Signs:
-Long face with prominent jaw and large ears
-Large testicles (macroorchidism) in postpubertal males
-Hypotonia
-Hypermobile joints
-Flat feet
-Sometimes, mitral valve prolapse.
Diagnostic Criteria:
-There are no formal diagnostic criteria in the sense of symptom thresholds, as FXS is a genetic disorder
-Diagnosis is confirmed by genetic testing that identifies mutations in the FMR1 gene
-Clinical suspicion should be high in individuals presenting with developmental delays, intellectual disability, and/or autism spectrum disorder, especially with a family history suggestive of X-linked inheritance.

Diagnostic Approach

History Taking:
-Detailed developmental history, including age of milestones
-Family history of intellectual disability, autism, or similar features, especially on the maternal side
-History of behavioral issues, including attention problems and social interaction difficulties
-Previous genetic testing results or known genetic conditions in the family
-Presence of seizures.
Physical Examination:
-Assess for characteristic facial features (long face, prominent jaw, large ears)
-Evaluate for hypotonia and joint hypermobility
-Check for macroorchidism in males
-Assess growth parameters
-Perform a thorough neurological examination
-Screen for cardiac murmurs suggesting mitral valve prolapse.
Investigations:
-Molecular genetic testing for the FMR1 gene is the gold standard
-This typically involves Southern blot analysis or repeat-primed PCR to detect the expanded CGG trinucleotide repeat in the promoter region of the FMR1 gene
-Analysis of repeat size distinguishes between normal, intermediate, premutation, and full mutation alleles
-Cytogenetic testing (karyotype) may be considered if other genetic abnormalities are suspected
-Electrophysiology (EEG) if seizures are present
-Echocardiography if mitral valve prolapse is suspected.
Differential Diagnosis:
-Other causes of intellectual disability: Down syndrome, Prader-Willi syndrome, Angelman syndrome, other chromosomal abnormalities
-Autism spectrum disorder: Idiopathic autism, Rett syndrome, other genetic causes of autism
-ADHD
-Other genetic syndromes with developmental delay and distinctive features.

Developmental Support And Management

Early Intervention:
-Multidisciplinary approach focusing on speech therapy, occupational therapy, and physical therapy
-Early intervention programs (Birth to Three) are critical for infants and toddlers with developmental delays
-Behavioral therapy and applied behavior analysis (ABA) for autistic features and behavior management
-Special education services tailored to individual learning needs.
Educational Support:
-Individualized Education Programs (IEPs) in school settings
-Support with academic learning, social skills development, and behavioral strategies
-Vocational training and life skills development for adolescents and adults.
Medical Management:
-Management of associated medical conditions: ADHD (stimulants or non-stimulants), anxiety (SSRIs), seizures (antiepileptic drugs like levetiracetam or valproate)
-Cardiac management for mitral valve prolapse if symptomatic
-No specific pharmacologic treatment exists for the core symptoms of FXS itself, but medications can manage comorbidities.
Family Support And Counseling:
-Genetic counseling for families to understand inheritance patterns, recurrence risks, and implications
-Support groups and resources for families of individuals with FXS
-Psychological support for parents and siblings to cope with the diagnosis and challenges.

Complications

Common Comorbidities:
-Autism spectrum disorder features are present in up to 60% of individuals with FXS
-ADHD is common, affecting a large proportion of affected individuals
-Anxiety disorders and mood disorders are prevalent in adolescents and adults
-Seizures occur in about 10-15%
-Mitral valve prolapse is a cardiac complication that may require management.
Behavioral Challenges: Aggression, self-injury, hyperactivity, social withdrawal, and sensory sensitivities can be significant challenges requiring tailored behavioral interventions.
Long Term Sequelae:
-Ongoing intellectual disability, persistent learning difficulties, and potential for chronic mental health issues require lifelong support and management
-Premutation carriers are at risk for Fragile X-associated Tremor/Ataxia Syndrome (FXTAS) and Fragile X-associated Primary Ovarian Insufficiency (FXPOI).

Prognosis

Factors Affecting Prognosis:
-Severity of intellectual disability, presence and severity of associated comorbidities (autism, ADHD, seizures), and access to early and consistent therapeutic interventions
-Family support and resources also play a role.
Outcomes:
-With comprehensive developmental support and management of comorbidities, individuals with FXS can achieve varying degrees of independence
-Many can learn to read, write, and perform daily living skills
-Social engagement and vocational potential vary widely
-Lifelong support is often necessary.
Follow Up:
-Regular developmental assessments throughout childhood and adolescence
-Annual check-ups with pediatrician to monitor growth, development, and manage comorbidities
-Specialist follow-up as needed for cardiology, neurology, psychiatry, and genetics
-Transition to adult healthcare services.

Key Points

Exam Focus:
-FXS is the most common inherited cause of intellectual disability and a leading genetic cause of ASD
-Caused by CGG repeat expansion in the FMR1 gene on the X chromosome
-Full mutation (>200 CGG repeats) leads to gene silencing
-Diagnosis is by molecular genetic testing (FMR1 gene analysis)
-Key features include intellectual disability, developmental delay, behavioral issues (ADHD, ASD traits), and characteristic physical features
-Management is supportive and multidisciplinary.
Clinical Pearls:
-Always consider FXS in children with unexplained intellectual disability or ASD, particularly with a suggestive family history
-Early intervention is paramount for improving developmental outcomes
-Be vigilant for associated medical comorbidities like seizures and mitral valve prolapse
-Remember premutation carriers are at risk for FXTAS and FXPOI.
Common Mistakes:
-Delayed diagnosis due to not considering genetic causes of developmental delay
-Inadequate multidisciplinary support
-Underestimating the importance of family genetic counseling
-Failing to screen for associated medical conditions
-Confusing FXS with idiopathic autism or ADHD without genetic workup.