Overview

Definition:
-HIV-exposed infants are newborns born to mothers living with HIV
-Prophylaxis refers to the use of antiretroviral drugs (ARVs) to prevent HIV infection in these infants
-Testing involves serial HIV testing to determine the infant's infection status.
Epidemiology:
-Globally, vertical transmission of HIV remains a significant concern, although rates have dramatically decreased with effective antiretroviral therapy (ART) in pregnant women
-In India, despite progress, continued vigilance and adherence to prophylaxis and testing protocols are crucial
-The risk of transmission without intervention can range from 15-45%.
Clinical Significance:
-Timely and appropriate prophylaxis significantly reduces the risk of perinatal HIV transmission, protecting infants from a chronic, life-limiting illness
-Early and accurate diagnosis through timely testing allows for prompt initiation of treatment, improving long-term outcomes and preventing the development of AIDS-related opportunistic infections and complications.

Prophylaxis Strategy

Perinatal Arv Prophylaxis:
-Antiretroviral prophylaxis for the infant is initiated as soon as possible after birth, ideally within 6-12 hours
-The regimen depends on the mother's viral load and ART status during pregnancy and delivery
-Three-drug prophylaxis is generally recommended for infants born to mothers with detectable viral load or unknown status.
Infant Arv Regimens:
-Standard prophylaxis for infants includes zidovudine (AZT) for 4-6 weeks
-For higher-risk infants (e.g., born to mothers with detectable viral load, premature birth, or intrapartum exposure), a three-drug regimen is used, typically including zidovudine, lamivudine, and a protease inhibitor (e.g., nelfinavir) or an integrase inhibitor (e.g., raltegravir), for 6 weeks
-Combination prophylaxis has demonstrated higher efficacy.
Duration And Discontinuation:
-Prophylaxis is typically continued for 4-6 weeks
-Discontinuation is considered after negative virologic testing at specific time points, and if all tests remain negative, the infant is considered uninfected
-If any test is positive, further evaluation and treatment are initiated.
Breastfeeding Recommendations:
-In resource-rich settings, breastfeeding is discouraged due to the risk of HIV transmission through breast milk
-However, in settings where safe alternatives are not feasible, exclusive breastfeeding for the first 6 months, along with continued infant ARV prophylaxis and maternal ART, may be considered
-Indian guidelines generally recommend formula feeding or avoidance of breastfeeding if feasible.

Testing Approach

Infant Virologic Testing:
-Virologic tests are used to detect HIV DNA or RNA and are the gold standard for diagnosing HIV infection in infants
-Initial testing is recommended at birth (for high-risk infants), 14-21 days, 1-2 months, and 4-6 months of age
-Nucleic acid amplification tests (NAATs) like PCR are preferred.
Diagnostic Thresholds:
-A positive virologic test (e.g., HIV-1 DNA PCR or HIV-1 RNA) at any time point after 48 hours of age is diagnostic of HIV infection
-Two separate positive virologic tests are required for definitive diagnosis, with the first test ideally performed at or after 14 days of age and the second at or after 1 month of age.
Serologic Testing:
-Antibody tests (ELISA, rapid antibody tests) are not reliable for diagnosing HIV infection in infants younger than 18 months due to the persistence of maternal antibodies
-These tests are used for initial screening of the mother and for confirmation of infection in older children.
Infant Follow Up Schedule:
-A comprehensive follow-up schedule is crucial
-This includes serial virologic testing at specific intervals (e.g., birth, 2 weeks, 1 month, 4 months) and antibody testing at 18 months for definitive confirmation of non-infection if all previous virologic tests were negative
-Clinical assessment for signs of HIV infection should also be performed at each visit.

Management Of Hiv Infected Infants

Initiation Of Art:
-Infants diagnosed with HIV infection should be initiated on antiretroviral therapy (ART) immediately
-Early initiation of ART is critical for immune reconstitution and preventing disease progression and mortality.
Pediatric Art Regimens:
-Recommended first-line ART regimens for infants and children typically include two nucleoside reverse transcriptase inhibitors (NRTIs) plus a non-nucleoside reverse transcriptase inhibitor (NNRTI) or a protease inhibitor (PI)
-Specific regimens are based on age, weight, and potential drug resistance
-For example, a common regimen may include zidovudine or tenofovir, lamivudine, and efavirenz or lopinavir/ritonavir.
Monitoring And Supportive Care:
-Regular monitoring of viral load, CD4 count, growth, development, and adherence to ART is essential
-Supportive care includes nutritional support, prophylaxis for opportunistic infections (e.g., Pneumocystis jirovecii pneumonia), and immunizations
-Management of co-infections and psychosocial support for the family are also vital components.

Challenges And Considerations

Adherence To Prophylaxis And Testing:
-Challenges include ensuring timely administration of prophylaxis, adherence to the complex testing schedule, and socioeconomic factors that may hinder access to care
-Education and support for caregivers are paramount.
Drug Resistance:
-Drug resistance can arise from suboptimal maternal ART adherence or infant prophylaxis
-Genotypic resistance testing may be indicated in cases of treatment failure or suspected resistance.
Stigma And Disclosure:
-Addressing stigma associated with HIV is important for families
-Disclosure of the infant's HIV status to caregivers and, at an appropriate age, to the child, requires careful and sensitive communication.
Resource Limitations:
-In resource-limited settings, access to rapid diagnostic tests, a wide range of ARVs, and skilled healthcare providers can be challenging
-Strategies to optimize care within available resources are crucial.

Key Points

Exam Focus:
-Understand the risk stratification for infant prophylaxis based on maternal viral load
-Memorize the recommended infant prophylaxis regimens and duration
-Recall the specific timing and types of virologic tests for infant diagnosis
-Know the criteria for definitive diagnosis of HIV infection in infants
-Differentiate between virologic and serologic testing in this age group.
Clinical Pearls:
-Initiate infant prophylaxis within 6-12 hours of birth, regardless of maternal ART status
-Ensure accurate documentation of maternal ART history and infant exposure
-Counsel caregivers thoroughly on the importance of adherence to prophylaxis and the testing schedule
-Coordinate with the mother's care team
-Consider gestational age and birth weight when selecting prophylaxis.
Common Mistakes:
-Delaying infant prophylaxis
-Relying solely on antibody tests for diagnosis in infants under 18 months
-Inadequate counseling on breastfeeding implications
-Failing to follow up with serial virologic testing as per guidelines
-Incorrect interpretation of early test results.