Overview

Definition:
-Giardiasis is an intestinal infection caused by the protozoan parasite Giardia lamblia (also known as Giardia intestinalis or duodenalis)
-Amebiasis is an intestinal infection caused by the protozoan parasite Entamoeba histolytica.
Epidemiology:
-Giardiasis is one of the most common waterborne parasitic diseases worldwide, with higher prevalence in areas with poor sanitation and unsafe drinking water
-Amebiasis is also globally distributed but is more endemic in tropical and subtropical regions with inadequate sanitation
-In children, both are significant causes of diarrheal illness, especially in daycare settings and communities with compromised hygiene.
Clinical Significance:
-Both infections can lead to acute or chronic diarrhea, malabsorption, and growth faltering in children, impacting their development and well-being
-Differentiating between them is crucial as treatment regimens and potential complications vary significantly, requiring appropriate diagnostic approaches for effective pediatric care and successful DNB/NEET SS examination preparation.

Clinical Presentation

Symptoms:
-Giardiasis: Abrupt onset of watery diarrhea, abdominal cramps, bloating, excessive gas, nausea, vomiting, anorexia, and foul-smelling stools
-Symptoms can be intermittent
-Amebiasis: Mild watery diarrhea to severe dysentery with blood and mucus in stools, abdominal pain (often crampy), fever, tenesmus
-Extraintestinal amebiasis (e.g., amebic liver abscess) can present with fever, hepatomegaly, and right upper quadrant pain.
Signs:
-Giardiasis: Dehydration, abdominal distension, tenderness
-Poor weight gain or weight loss in chronic cases
-Amebiasis: Fever, tenderness over the liver if abscess is present, signs of dehydration, severe abdominal tenderness and guarding in invasive disease.
Diagnostic Criteria:
-Diagnosis is primarily based on identification of the parasite in stool samples
-For Giardiasis, this involves microscopic examination for cysts or trophozoites, antigen detection assays (ELISA), or molecular methods
-For Amebiasis, stool microscopy for E
-histolytica cysts or trophozoites (often with characteristic ingested red blood cells), antigen detection, serology (for invasive disease), and PCR are used
-Imaging (ultrasound, CT) is crucial for suspected extraintestinal amebiasis.

Diagnostic Approach

History Taking:
-Key history points include travel history, recent consumption of untreated water or food, exposure to contaminated sources (e.g., daycare, swimming pools), duration and character of diarrhea (watery vs
-bloody), associated symptoms like fever, vomiting, abdominal pain, and signs of malabsorption or dehydration
-Ask about prior parasitic infections.
Physical Examination:
-Assess hydration status carefully
-Perform a thorough abdominal examination for tenderness, distension, guarding, and organomegaly (especially liver for amebiasis)
-Evaluate for signs of malnutrition or growth faltering.
Investigations:
-Stool microscopy for ova and parasites (O&P) is the initial test for both
-multiple samples may be needed
-Antigen detection tests (e.g., Giardia-specific antigen, E
-histolytica antigen) are more sensitive
-PCR for Giardia or E
-histolytica is highly specific
-For suspected invasive amebiasis, consider serological tests (e.g., indirect hemagglutination assay - IHA) and imaging of the liver
-Complete blood count may show eosinophilia in Giardiasis and leukocytosis in Amebiasis.
Differential Diagnosis:
-Other causes of pediatric diarrhea: bacterial gastroenteritis (Salmonella, Shigella, E
-coli, Campylobacter), viral gastroenteritis (Rotavirus, Norovirus), inflammatory bowel disease, malabsorption syndromes (celiac disease, lactose intolerance), irritable bowel syndrome, and other parasitic infections (Cryptosporidiosis, Cyclospora)
-Invasive amebiasis must be differentiated from pyogenic liver abscesses.

Management

Initial Management:
-Primary goal is rehydration and nutritional support
-Oral rehydration solution (ORS) is the mainstay for mild to moderate dehydration
-Severe dehydration requires intravenous fluid resuscitation
-Identify and treat nutritional deficiencies, especially in cases of chronic diarrhea or malabsorption.
Medical Management:
-Giardiasis: First-line treatment is usually Tinidazole (5-10 mg/kg/day once daily for 3 days) or Metronidazole (15 mg/kg/day divided into 3 doses for 5-7 days, max 750 mg/day)
-Alternative agents include Nitazoxanide (100-200 mg BID for 3 days in children >12 years or 5 mg/kg BID for 3 days in children <12 years)
-Amebiasis: Asymptomatic cyst passers: Diloxanide furoate (20 mg/kg/day divided into 3 doses for 10 days) or Iodoquinol
-Symptomatic intestinal amebiasis: Metronidazole (35-50 mg/kg/day divided into 3 doses for 10 days, max 2-3 g/day), followed by a luminal agent (Diloxanide furoate or Iodoquinol)
-Invasive amebiasis (extraintestinal or severe intestinal): Metronidazole, followed by a luminal agent
-For amebic liver abscess, drainage may be necessary along with medical therapy.
Surgical Management:
-Rarely indicated for intestinal amebiasis unless complications like perforation or toxic megacolon occur
-Surgical intervention may be required for large or complicated amebic liver abscesses that do not respond to medical management or have complications like rupture.
Supportive Care:
-Maintain adequate fluid and electrolyte balance
-Monitor for signs of dehydration and complications
-Ensure appropriate nutritional intake, considering temporary lactose restriction if symptoms persist
-Educate caregivers on hygiene practices to prevent transmission.

Complications

Early Complications:
-Giardiasis: Severe dehydration, electrolyte imbalances, reactive arthritis (rare)
-Amebiasis: Profuse bloody diarrhea, toxic megacolon, bowel perforation, peritonitis, fulminant amebic colitis
-Early extraintestinal complications include amebic liver abscess, amebic lung abscess, or amebic brain abscess.
Late Complications:
-Giardiasis: Chronic diarrhea, malabsorption, weight loss, failure to thrive, post-infectious irritable bowel syndrome
-Amebiasis: Residual liver abscesses, fistulas, strictures, appendicitis (due to amoebomas), increased risk of colorectal cancer with chronic infections.
Prevention Strategies:
-Emphasize good personal hygiene (handwashing), safe water practices (boiling, filtering, treating water), proper food handling and preparation, and effective sanitation systems
-Vaccination is not available for either infection
-Prompt diagnosis and treatment of infected individuals are crucial to limit spread.

Prognosis

Factors Affecting Prognosis:
-Host immune status, nutritional status, severity of infection, timeliness of diagnosis and treatment, and presence of complications
-Children with underlying immunocompromise or malnutrition have a poorer prognosis.
Outcomes:
-With appropriate antiparasitic treatment and supportive care, most children with uncomplicated giardiasis and intestinal amebiasis recover fully
-Chronic or complicated cases, especially with extraintestinal involvement, can have a prolonged recovery and may require multidisciplinary management.
Follow Up:
-Follow-up is important for children with chronic symptoms, malabsorption, or failure to thrive
-Repeat stool examinations may be necessary to confirm eradication
-Monitor growth and development closely
-Patients with amebic liver abscesses require prolonged follow-up and imaging to ensure complete resolution and rule out recurrence.

Key Points

Exam Focus:
-Distinguishing clinical features and first-line treatment options for giardiasis vs
-amebiasis in children
-Management of asymptomatic cyst passers versus symptomatic patients in amebiasis
-Recognition and initial management of amebic liver abscess
-Importance of luminal agents after systemic treatment for invasive amebiasis
-Role of hydration and nutrition in all pediatric diarrheal illnesses.
Clinical Pearls:
-Always consider both Giardia and Entamoeba in children with persistent or recurrent diarrhea, especially with a history of travel or exposure to contaminated water
-A high index of suspicion for amebiasis is warranted in endemic areas or in immunocompromised individuals
-Remember that some patients with E
-histolytica can be asymptomatic carriers but still excrete infectious cysts
-Metronidazole is a cornerstone for amebiasis treatment but does not eradicate cysts
-hence, a luminal agent is essential.
Common Mistakes:
-Mistaking amebic dysentery for bacterial dysentery and using antibiotics instead of antiparasitics
-Failing to treat asymptomatic E
-histolytica carriers, leading to continued transmission
-Inadequate treatment of amebic liver abscesses (e.g., omitting luminal agents or failing to drain)
-Overlooking dehydration and nutritional deficiencies in pediatric diarrheal illnesses
-Relying on a single stool O&P examination for diagnosis, as sensitivity can be low.