Overview

Definition:
-Thalassemia major, also known as Cooley's anemia, is a severe inherited blood disorder characterized by reduced or absent synthesis of beta-globin chains, leading to ineffective erythropoiesis and severe, life-threatening anemia
-It is an autosomal recessive disorder.
Epidemiology:
-Most common inherited blood disorder globally, with a high carrier frequency in Mediterranean, Middle Eastern, South Asian, and Southeast Asian populations
-In India, carrier rates vary significantly by region, with higher prevalence in certain ethnic groups
-Incidence of major forms necessitates regular transfusions.
Clinical Significance:
-Thalassemia major is a chronic, lifelong condition requiring intensive management
-Without appropriate treatment, patients develop severe anemia, growth retardation, skeletal deformities, extramedullary hematopoiesis, and life-threatening iron overload from frequent blood transfusions
-Effective management is crucial for survival and quality of life.

Clinical Presentation

Symptoms:
-Severe anemia presenting in infancy (typically 3-6 months of age)
-Pallor
-Jaundice
-Poor feeding
-Failure to thrive
-Abdominal distension due to splenomegaly and hepatomegaly
-Bone deformities (frontal bossing, maxillary prominence, thinning of long bones)
-Delayed puberty
-Pathological fractures.
Signs:
-Marked pallor
-Icterus
-Hepatosplenomegaly
-Characteristic facial dysmorphorphism (thalassemic facies)
-Skeletal abnormalities (e.g., "chipmunk facies", "hair-on-end" appearance on skull X-ray)
-Growth retardation
-Signs of iron overload: cardiac dysfunction (arrhythmias, heart failure), hepatic dysfunction (fibrosis, cirrhosis), endocrine dysfunction (diabetes mellitus, hypogonadism, hypothyroidism, hypoparathyroidism).
Diagnostic Criteria:
-Clinical presentation of severe anemia in infancy
-Confirmation by hemoglobin electrophoresis demonstrating absence or severe reduction of adult hemoglobin (HbA) and a high proportion of fetal hemoglobin (HbF)
-Genetic testing (beta-globin gene sequencing) can identify specific mutations
-Diagnosis is often made based on typical clinical and hematological findings.

Diagnostic Approach

History Taking:
-Detailed family history of anemia or similar blood disorders
-Age of onset of symptoms
-History of blood transfusions and response
-Previous investigations
-Growth parameters
-Signs of complications like fever, bone pain, or abdominal pain.
Physical Examination:
-Assessment of vital signs
-Thorough assessment for pallor and jaundice
-Palpation for hepatosplenomegaly
-Detailed examination of skeletal system for deformities
-Assessment of growth and development
-Examination for signs of endocrine dysfunction.
Investigations:
-Complete Blood Count (CBC): severe microcytic hypochromic anemia (Hb < 7 g/dL), high reticulocyte count, anisopoikilocytosis
-Peripheral smear: target cells, basophilic stippling, nucleated red blood cells
-Hemoglobin electrophoresis: HbF > 90%, absence of HbA, presence of HbA2
-Serum ferritin: elevated, indicating iron overload
-Liver function tests (LFTs), Renal function tests (RFTs), Thyroid function tests (TFTs), Serum amylase, Glycosylated hemoglobin (HbA1c)
-Echocardiogram for cardiac function and iron deposition
-MRI of liver and heart for quantitative iron assessment.
Differential Diagnosis:
-Other severe microcytic hypochromic anemias (e.g., iron deficiency anemia - typically responds to iron, megaloblastic anemia - different red cell morphology, anemia of chronic disease)
-Sickle cell disease (different hemoglobin electrophoresis pattern)
-Alpha thalassemia major (rare, hydrops fetalis)
-Congenital dyserythropoietic anemias.

Management

Initial Management:
-Immediate initiation of regular blood transfusions (hypertransfusion regimen) to maintain pre-transfusion hemoglobin levels between 9-10.5 g/dL and post-transfusion levels of 12-14.5 g/dL
-This suppresses ineffective erythropoiesis and reduces bone marrow expansion.
Medical Management:
-Iron Chelation Therapy: Essential to prevent and manage iron overload
-- Deferoxamine: Subcutaneous or intravenous infusion, typically 5-7 nights/week
-Requires adherence and can cause injection site reactions, hearing and vision abnormalities
-- Deferasirox: Oral iron chelator, once daily
-Dosage adjusted based on serum ferritin levels and body weight
-Common side effects include gastrointestinal upset, rash, and renal impairment
-- Deferiprone: Oral iron chelator, 3 times daily
-Primarily removes cardiac iron
-Can cause agranulocytosis and neutropenia, requiring regular blood count monitoring
-Combination therapy with deferasirox and deferiprone is often used for severe iron overload, particularly cardiac iron
-Dosing for chelators is critical and guided by serum ferritin and organ iron levels
-Folic acid supplementation (1 mg/day) is standard for patients on transfusions
-Hydroxyurea may be used in select cases to increase HbF levels but is less effective in beta-thalassemia major.
Surgical Management:
-Splenectomy: May be considered in patients with massive splenomegaly leading to hypersplenism (increasing transfusion requirements or causing significant discomfort), but it increases the risk of overwhelming post-splenectomy infection (OPSI) and thromboembolic events
-Vaccination against encapsulated organisms is crucial post-splenectomy
-Bone marrow or stem cell transplantation (BMT/SCT) is the only curative option, indicated for appropriately matched sibling donors
-Allogeneic hematopoietic stem cell transplantation is the preferred treatment if a suitable donor is available.
Supportive Care:
-Nutritional support to ensure adequate growth
-Management of complications like osteoporosis, diabetes, hypothyroidism, cardiac dysfunction
-Psychological support for patients and families
-Regular monitoring of growth parameters, organ function, and iron levels.

Complications

Early Complications:
-Transfusion reactions (febrile, allergic, hemolytic)
-Volume overload
-Iron overload due to transfusions and increased absorption
-Infection from transfusions (Hepatitis B, C, HIV - significantly reduced with modern screening).
Late Complications:
-Severe iron overload leading to cardiac failure, arrhythmias, liver cirrhosis, diabetes mellitus, hypogonadism, hypothyroidism, osteoporosis, and bone fractures
-Extramedullary hematopoiesis causing mass effect (e.g., spinal cord compression)
-Thromboembolic events
-Delayed puberty and infertility.
Prevention Strategies:
-Strict adherence to regular blood transfusions and effective iron chelation therapy is paramount
-Pre-transfusion screening for infections
-Regular monitoring of iron levels (serum ferritin, MRI) and organ function
-Vaccination against common infections, especially in splenectomized patients.

Prognosis

Factors Affecting Prognosis:
-Age of initiation of adequate transfusions and chelation therapy
-Efficacy and adherence to chelation
-Development of organ damage from iron overload
-Presence of complications
-Availability of stem cell transplantation
-Genetic mutation type.
Outcomes:
-With optimal transfusions and chelation, survival into adulthood is now common, with many patients achieving good quality of life
-However, long-term complications of iron overload can still occur
-Stem cell transplantation offers a potential cure.
Follow Up:
-Lifelong follow-up is essential
-Regular clinical assessment, hematological monitoring, serum ferritin levels, echocardiograms, liver MRI, endocrine assessments, and bone density scans
-Management of comorbidities and complications.

Key Points

Exam Focus:
-The cornerstone of management for thalassemia major is regular blood transfusions and iron chelation therapy
-Understand the indications and side effects of deferoxamine, deferasirox, and deferiprone
-Recognize signs and symptoms of iron overload in various organs
-Know the diagnostic criteria and typical hemoglobin electrophoresis findings.
Clinical Pearls:
-Always check for signs of iron overload, especially cardiac and hepatic involvement, even in well-managed patients
-Dosing of chelators is individualized and requires careful monitoring
-Consider BMT/SCT as a curative option if a suitable donor exists
-Vaccinate patients who have undergone splenectomy.
Common Mistakes:
-Delaying initiation of transfusions or chelation
-Inadequate chelation therapy leading to progressive iron overload
-Underestimating the cardiac impact of iron overload
-Failing to monitor for rare but serious side effects of chelators (e.g., agranulocytosis with deferiprone).