Definition/General

Introduction:
-A fibroadenoma is the most common benign tumor of the female breast
-It is a biphasic tumor composed of a proliferation of both epithelial and stromal elements.
Origin: It arises from the terminal duct-lobular unit (TDLU).
Classification:
-Fibroadenomas are classified as benign fibroepithelial tumors
-Variants include juvenile fibroadenoma and complex fibroadenoma.
Epidemiology:
-They are most common in young women, with a peak incidence in the 20s and 30s
-They can be multiple and bilateral in up to 20% of cases.

Clinical Features

Presentation:
-Presents as a painless, firm, mobile, well-circumscribed, rubbery mass
-They are often described as a "breast mouse" due to their mobility.
Symptoms:
-A painless, mobile lump is the most common symptom
-They can increase in size during pregnancy and lactation.
Risk Factors: They are hormonally responsive and can grow in response to estrogen.
Screening:
-On mammography, they appear as a well-circumscribed, oval mass that may have "popcorn-like" calcifications
-On ultrasound, they are a well-defined, hypoechoic mass.

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Gross Description

Appearance:
-A well-circumscribed, encapsulated, firm, gray-white mass that bulges from the cut surface.
Characteristics: The cut surface is whorled and may have slit-like spaces.
Size Location:
-Size is variable, but they are typically 1-3 cm
-They can occur anywhere in the breast.
Multifocality: Can be multiple and bilateral.

Microscopic Description

Histological Features:
-A biphasic tumor with a proliferation of benign glands and stroma
-The glands are lined by a double layer of epithelial and myoepithelial cells
-The stroma is typically bland and fibroblastic.
Cellular Characteristics:
-The epithelial and stromal cells are benign, with no atypia or mitotic activity.
Architectural Patterns:
-Two main patterns are described: intracanalicular (stroma compresses the glands into slit-like spaces) and pericanalicular (stroma surrounds open, rounded glands)
-Most fibroadenomas have a mixed pattern.
Grading Criteria: This is a benign lesion.

Immunohistochemistry

Positive Markers:
-The epithelial cells are positive for cytokeratins and ER
-The myoepithelial cells are positive for p63 and calponin
-The stromal cells are positive for vimentin and CD34.
Negative Markers: Not typically required for diagnosis.
Diagnostic Utility: IHC is not usually necessary for diagnosis but can be used to confirm the presence of a myoepithelial layer.
Molecular Subtypes: Molecular subtyping is not relevant for this benign condition.

Molecular/Genetic

Genetic Mutations: Recurrent mutations in the MED12 gene are found in a high percentage of fibroadenomas.
Molecular Markers: No specific molecular markers are routinely used for diagnosis.
Prognostic Significance:
-Fibroadenomas are benign
-Simple fibroadenomas are not associated with an increased risk of breast cancer
-Complex fibroadenomas (which have features like cysts >3mm, sclerosing adenosis, epithelial calcifications, or papillary apocrine change) are associated with a small increased risk.
Therapeutic Targets:
-Treatment is usually conservative (observation)
-Excision may be performed for large or symptomatic lesions, or if there is diagnostic uncertainty.

Differential Diagnosis

Similar Entities:
-Phyllodes tumor
-Tubular adenoma.
Distinguishing Features:
-Phyllodes tumors have a more cellular stroma, often with a leaf-like architecture, and can show stromal atypia and mitoses
-Tubular adenomas are composed almost exclusively of small, closely packed tubules.
Diagnostic Challenges: The main challenge is distinguishing a fibroadenoma from a benign phyllodes tumor on a core needle biopsy, as the stromal cellularity can be variable.
Rare Variants:
-Juvenile fibroadenoma is a rapidly growing variant seen in adolescents
-Giant fibroadenoma is a term for fibroadenomas >5 cm or >500g.

Sample Pathology Report

Template Format

Sample Pathology Report

Complete Report: This is an example of how the final pathology report should be structured for this condition.

Specimen Information

[specimen type], measuring [size] cm in greatest dimension

Diagnosis

[diagnosis name]

Classification

Classification: [classification system] [grade/type]

Histological Features

Shows [architectural pattern] with [nuclear features] and [mitotic activity]

Size and Extent

Size: [X] cm, extent: [local/regional/metastatic]

Margins

Margins are [involved/uninvolved] with closest margin [X] mm

Lymphovascular Invasion

Lymphovascular invasion: [present/absent]

Lymph Node Status

Lymph nodes: [X] positive out of [X] examined

Special Studies

IHC: [marker]: [result]

Molecular: [test]: [result]

[other study]: [result]

Final Diagnosis

Final diagnosis: [complete diagnosis]