Definition/General

Introduction:
-Lobular carcinoma in situ (LCIS) is a non-invasive breast lesion characterized by the proliferation of abnormal cells within the breast lobules
-It is considered a risk factor for developing invasive breast cancer in both breasts, rather than a direct precursor lesion.
Origin:
-LCIS arises from the terminal duct-lobular unit (TDLU)
-It is characterized by the loss of the cell adhesion molecule E-cadherin, which leads to the discohesive growth pattern.
Classification:
-LCIS is part of the spectrum of lobular neoplasia, which also includes atypical lobular hyperplasia (ALH)
-Variants of LCIS include the classic, pleomorphic, and florid types.
Epidemiology:
-LCIS is most often an incidental finding in breast biopsies performed for other reasons
-It is most common in premenopausal women.

Clinical Features

Presentation:
-LCIS is typically asymptomatic and does not form a palpable mass or produce mammographic calcifications
-It is usually an incidental finding.
Symptoms: Asymptomatic.
Risk Factors: The risk factors are similar to those for invasive breast cancer.
Screening:
-LCIS is not typically detected by mammography
-It is found incidentally on biopsy.

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Gross Description

Appearance: There are no specific gross findings for LCIS.
Characteristics: Gross findings are not specific for this microscopic diagnosis.
Size Location: Gross findings are not specific for this microscopic diagnosis.
Multifocality: LCIS is often multifocal and bilateral.

Microscopic Description

Histological Features:
-The lobules are expanded and filled with a monotonous population of small, discohesive cells
-The cells have scant cytoplasm and round, uniform nuclei
-The underlying lobular architecture is preserved.
Cellular Characteristics:
-The cells are small and uniform with round nuclei and inconspicuous nucleoli
-Intracytoplasmic mucin vacuoles (signet ring cells) can be seen
-Mitotic activity is low.
Architectural Patterns:
-The key feature is the filling and distension of the acini of the TDLU by a discohesive population of cells
-Pagetoid spread into ducts can occur.
Grading Criteria:
-Classic LCIS is considered a low-grade lesion
-Pleomorphic LCIS has larger cells with more nuclear atypia and is considered a more significant lesion.

Immunohistochemistry

Positive Markers:
-The tumor cells are positive for ER and PR in almost all cases
-They are positive for cytokeratins.
Negative Markers:
-The hallmark of LCIS is the complete loss of E-cadherin expression
-HER2 is negative.
Diagnostic Utility:
-IHC for E-cadherin is essential for diagnosis and to differentiate LCIS from low-grade DCIS
-A negative E-cadherin stain confirms a lobular lesion.
Molecular Subtypes: Not applicable in the same way as for invasive cancer.

Molecular/Genetic

Genetic Mutations:
-The key genetic event is the loss of the CDH1 gene (which codes for E-cadherin), often due to a mutation or promoter methylation.
Molecular Markers: Loss of E-cadherin is the key molecular marker.
Prognostic Significance:
-LCIS is a risk factor for subsequent invasive carcinoma, which can be ductal or lobular, in either breast
-The risk is about 1% per year
-Pleomorphic LCIS may have a higher risk of progression.
Therapeutic Targets:
-Management is controversial and ranges from observation to risk-reducing medication (e.g., tamoxifen) to bilateral mastectomy in high-risk patients.

Differential Diagnosis

Similar Entities:
-Atypical lobular hyperplasia (ALH)
-Low-grade DCIS
-Invasive lobular carcinoma.
Distinguishing Features:
-ALH is distinguished from LCIS by the degree of acinar involvement
-in ALH, the acini are not completely filled and distended
-Low-grade DCIS is E-cadherin positive
-Invasive lobular carcinoma shows infiltration of the stroma.
Diagnostic Challenges:
-The distinction between ALH and LCIS can be subjective
-Differentiating LCIS with pagetoid spread into ducts from DCIS can be challenging without E-cadherin IHC.
Rare Variants: Pleomorphic LCIS and florid LCIS are important variants to recognize due to their potential for being associated with invasive carcinoma.

Sample Pathology Report

Template Format

Sample Pathology Report

Complete Report: This is an example of how the final pathology report should be structured for this condition.

Specimen Information

[specimen type], measuring [size] cm in greatest dimension

Diagnosis

[diagnosis name]

Classification

Classification: [classification system] [grade/type]

Histological Features

Shows [architectural pattern] with [nuclear features] and [mitotic activity]

Size and Extent

Size: [X] cm, extent: [local/regional/metastatic]

Margins

Margins are [involved/uninvolved] with closest margin [X] mm

Lymphovascular Invasion

Lymphovascular invasion: [present/absent]

Lymph Node Status

Lymph nodes: [X] positive out of [X] examined

Special Studies

IHC: [marker]: [result]

Molecular: [test]: [result]

[other study]: [result]

Final Diagnosis

Final diagnosis: [complete diagnosis]