Definition/General

Introduction:
-Microglandular adenosis (MGA) is a rare benign proliferative breast lesion characterized by a haphazard proliferation of small, round, open glands
-A key feature is the absence of a myoepithelial layer, which can cause it to be mistaken for invasive carcinoma.
Origin: It arises from the terminal duct-lobular unit (TDLU).
Classification:
-MGA is classified as a benign proliferative breast lesion
-It can be associated with atypia (atypical MGA) and can be a precursor to carcinoma arising in MGA.
Epidemiology:
-It is a rare lesion, most often found in women in their 40s and 50s.

Clinical Features

Presentation: MGA can present as a palpable mass or be an incidental finding on a biopsy performed for other reasons.
Symptoms: Usually asymptomatic, but can form a palpable lump.
Risk Factors: There are no well-established risk factors.
Screening: Mammographic findings are non-specific and can include a mass, architectural distortion, or calcifications.

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Gross Description

Appearance:
-It can form a firm, ill-defined, yellow-tan mass.
Characteristics: The size is variable.
Size Location: Can occur anywhere in the breast.
Multifocality: Can be multifocal.

Microscopic Description

Histological Features:
-The lesion is composed of a proliferation of small, round, uniform glands infiltrating the stroma and adipose tissue
-The glands have open lumina that often contain eosinophilic, colloid-like secretions
-The key feature is the absence of a myoepithelial layer.
Cellular Characteristics:
-The cells are cuboidal with scant, eosinophilic or vacuolated cytoplasm
-The nuclei are small, round, and uniform with inconspicuous nucleoli
-Mitotic activity is low.
Architectural Patterns: The glands are haphazardly arranged and infiltrate the stroma in a pattern that can mimic tubular carcinoma.
Grading Criteria:
-This is a benign lesion
-Atypical MGA shows increased cytological atypia.

Immunohistochemistry

Positive Markers:
-The epithelial cells are positive for S100 and low molecular weight cytokeratins
-They are also positive for collagen IV and laminin, which highlights a continuous basement membrane around the glands.
Negative Markers:
-The cells are negative for ER, PR, and HER2
-Importantly, they are negative for myoepithelial markers (e.g., p63, calponin), which is a diagnostic pitfall.
Diagnostic Utility:
-IHC is crucial for diagnosis
-The combination of S100 positivity, ER negativity, and absence of myoepithelial markers is characteristic of MGA and helps distinguish it from tubular carcinoma.
Molecular Subtypes: Molecular subtyping is not relevant for this benign condition.

Molecular/Genetic

Genetic Mutations: Not well characterized.
Molecular Markers: No specific molecular markers are routinely used for diagnosis.
Prognostic Significance:
-MGA is a risk factor for breast cancer
-It can be a precursor to a rare type of triple-negative carcinoma that arises in MGA.
Therapeutic Targets: Surgical excision is recommended to exclude associated atypia or carcinoma.

Differential Diagnosis

Similar Entities:
-Tubular carcinoma
-Sclerosing adenosis.
Distinguishing Features:
-Tubular carcinoma is ER-positive and S100-negative
-Sclerosing adenosis has a myoepithelial layer
-The IHC profile of MGA (S100+, ER-, myoepithelial markers-) is very helpful in the differential diagnosis.
Diagnostic Challenges:
-The main challenge is distinguishing MGA from tubular carcinoma, especially on a small biopsy
-The absence of a myoepithelial layer in a benign lesion is a major diagnostic pitfall.
Rare Variants: Atypical MGA is a variant with increased cytological atypia.

Sample Pathology Report

Template Format

Sample Pathology Report

Complete Report: This is an example of how the final pathology report should be structured for this condition.

Specimen Information

[specimen type], measuring [size] cm in greatest dimension

Diagnosis

[diagnosis name]

Classification

Classification: [classification system] [grade/type]

Histological Features

Shows [architectural pattern] with [nuclear features] and [mitotic activity]

Size and Extent

Size: [X] cm, extent: [local/regional/metastatic]

Margins

Margins are [involved/uninvolved] with closest margin [X] mm

Lymphovascular Invasion

Lymphovascular invasion: [present/absent]

Lymph Node Status

Lymph nodes: [X] positive out of [X] examined

Special Studies

IHC: [marker]: [result]

Molecular: [test]: [result]

[other study]: [result]

Final Diagnosis

Final diagnosis: [complete diagnosis]